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H₂-driven reduction of CO₂ to formate using bacterial plasma membranes

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Bacterial membranes shield the intracellular compartment by selectively allowing unwanted substances to enter in, which in turn reduces overall catalytic efficiency. This report presents a model system using the isolated plasma membranes of Citrobacter sp. S-77 that harbor oxygen-stable [NiFe]hydrogenase and [Mo]formate dehydrogenase, which are integrated into a natural catalytic nanodevice through an electron transfer relay. This naturally occurring nanodevice exhibited selectivity and efficiency in catalyzing the H2-driven conversion of CO2 to formate with the rate of 817 mmol·L–1·gprotein–1·h−1 under mild conditions of 30 °C, pH 7.0, and 0.1 MPa. When the isolated plasma membranes of Citrobacter sp. S-77 was immobilized with multi-walled carbon nanotubes and encapsulated in hydrogel beads of gellan-gum cross-linked with calcium ions, the catalyst for formate production remained stable over 10 repeated uses. This paper reports the first case of efficient and selective formate production from H2 and CO2 using bacterial plasma membranes.

Mohammad Moniruzzaman, Hung Khac Nguyen, Yu Kiyasu, Takumi Hirose, Yuya Handa, Taro Koide, Seiji Ogo and  Ki-Seok Yoon, H₂-driven reduction of CO₂ to formate using bacterial plasma membranes, Bioresource Technology, 2023

https://doi.org/10.1016/j.biortech.2023.129921

 

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H₂-driven reduction of CO₂ to formate using bacterial plasma membranes